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Biochemistry

Hands-on biochemistry training covering biomolecule and phytochemical analysis, SDS-PAGE, spectrophotometry, chromatography and protein purification.

  • RIB - REGIONAL INSTITUTE OF BIOTECHNOLOGY
  • Bhubaneswar, Odisha
  • 1 Oct, 202631 Oct, 2026

Key dates

Apply opens26 Aug
Apply by30 Sep
Starts1 Oct
Ends31 Oct

Internship

  • ModeIn-person

Commitment

  • Duration1 month

Participation

  • Fee₹5,000
  • Seats10 seats

About this internship

This one-month hands-on training program in Biochemistry is designed to provide practical exposure to fundamental biochemical laboratory techniques and analytical methods. Participants will learn reagent preparation, basic laboratory instrument handling, biomolecule estimation, chromatographic techniques, electrophoresis and spectrophotometric analysis.

The training covers qualitative and quantitative analysis of proteins, carbohydrates, lipids and various phytochemicals. Participants will also gain practical knowledge of SDS-PAGE, protein characterization, enzyme activity determination, thin-layer chromatography and alcohol estimation.

The program further introduces recombinant protein expression in E. coli and purification using affinity chromatography, providing exposure to important techniques used in modern biotechnology and biochemical research.

The training is suitable for students interested in Biochemistry, Biotechnology, Life Sciences and related laboratory-based fields.

What you'll learn

  • Hands-on training in Molarity, Normality, Percentage and Reagent Preparation
  • Practical experience in basic laboratory instrument handling
  • Qualitative detection of phytochemicals and macronutrients such as proteins and carbohydrates
  • Extraction and estimation of phytochemicals including alkaloids, flavonoids, tannins, terpenoids, saponins, chlorophyll and more
  • Protein extraction and estimation using Bradford, Biuret and Lowry methods
  • Carbohydrate extraction and estimation using the Anthrone method
  • Reducing sugar estimation using the DNS method
  • Lipid extraction and estimation from plant and animal samples
  • SDS-PAGE: gel casting, apparatus assembly and protein characterization
  • Standard operating procedures and practical use of spectrophotometers
  • Determination of alpha-amylase activity in saliva
  • Detection of organophosphate pesticides using Thin-Layer Chromatography (TLC)
  • Practical exposure to recombinant protein expression in E. coli
  • Protein purification using affinity chromatography
  • Alcohol estimation using the potassium dichromate method
  • Development of practical skills in biochemical analysis, phytochemical screening and laboratory techniques

Topics covered

Biochemistry FundamentalsReagent PreparationLaboratory TechniquesProtein BiochemistryCarbohydrate BiochemistryLipid BiochemistryPhytochemicalsProtein EstimationCarbohydrate EstimationEnzyme AssaySDS-PAGESpectrophotometryThin Layer ChromatographyRecombinant DNA TechnologyRecombinant Protein ExpressionProtein PurificationAffinity Chromatography

Instruments used

MicropipetteAnalytical/Precision BalancepH MeterSpectrophotometerCentrifugeSDS-PAGE Electrophoresis UnitGel Casting ApparatusTLC ChamberIncubator / Shaking IncubatorChromatography ColumnGel Documentation System

Who you'll work with

SH

Dr. Shaikh Nausad Hossain

Director, Regional Institute of Biotechnology

Where it happens

RIB - REGIONAL INSTITUTE OF BIOTECHNOLOGY

Ground Floor, Regional Institute of Biotechnology, Plot No.266, Damana Square - Damana School Rd, opposite of Hallmark Showroom, Damana, Chandrasekharpur, Bhubaneswar, Odisha 751016, India